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To investigate the hydrolytic pattern of mannan polymers, reactions with various mannan substrates were evaluated based on TLC method using recombinant ManKs_4-555, and results are shown in Fig. 5.
For this, we set up modified ELISA assay method using recombinant AIMP1 and gp96 proteins.
Both plants and plant tissue cultures are amenable to a rapid, scalable and low-cost gene delivery method using recombinant clones of Agrobacterium tumefaciens.
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This elegant method uses recombinant antibodies engineered with the antigen incorporated as a fusion protein that, in addition to targeting the antigen to antigen presenting cells (APCs), has the advantages of a standardized manufacturing process typical for monoclonal antibodies and a well-defined regulatory path.
There have been several attempts to develop effective LLO purification methods using recombinant Escherichia coli strains [ 5- 9].
Nucleosomes were assembled using standard salt-gradient dialysis methods using recombinant Xenopus laevis histones and PCR-generated DNA fragments containing either the 601 (Thastrom et al, 1999) or MMTV NucA positioning sequences, as described previously (Ferreira et al, 2007).
The kinase assays were performed as reported in Materials and Methods using recombinant B-Raf (wt B-Raf or constitutively active mutant B-Raf V600E) as well as C-Raf DD (constitutively active C-Raf DD) purified from baculovirus-infected Sf9 cells as described previously.
The acetylcholinesterase inhibition effect of the isolated compounds (1 4) was assessed by Ellman's spectrophotometric method using human recombinant AChE with tacrine as reference compound.
However, it may be possible to reduce the cytotoxic effects and develop a relatively efficient gene delivery method to butterfly wings using recombinant baculovirus vectors.
In another method, cinnamic acid production using recombinant E. coli strains under alkaline conditions yielded about 15% cinnamic acid (Ben-Bassat et al. 2005).
The catalytic domain of the YscN protein was made using recombinant methods and its ATPase activity was characterized in vitro.
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method using deep
method using circular
method using natural
method using whole
method using statistical
method using supercritical
method using pure
method using conventional
method using fuzzy
method using standard
method using dynamic
method using static
method using aqueous
method using real
method using linear
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