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Changes in the structure of actin microfilaments were evaluated using the method of fluorescent microscopy and by using an LSM 780 (Carl Zeiss, Oberkochen, Germany) confocal microscope.
One method of fluorescent detection of free Mn2+ described by the Canary laboratory in 2009 involves an ion displacement reaction.
The method of fluorescent quantification at the skin surface was used to quantify the penetration of δ-aminolevulinic acid (ALA) and its methyl ester into the tissue [ 98].
A tumor was considered to be HER2 positive if the primary or metastatic tumor was scored 3+ by HER2 immunohistochemistry (IHC) or by amplification of the HER2 gene by the method of fluorescent in situ hybridization (FISH).
In order to reliably quantify the differently sized amplicons, we employed a cost-efficient method of fluorescent labeling of PCR products with the common M13-40-NED M13-40-NED M13-40-NED
To address these practical issues, we investigated whether we could use blue-green derived fluorophores from a third species, aequorea coerulescens, derived from the wild-type green fluorescent protein, aceGFP (16), as a fluorophore to practically co-image with eGFP and mRFP by the method of fluorescent widefield microscopy using a xenon light source.
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The formation of coordination complexes is a relatively straightforward method of constructing fluorescent and colorimetric chemosensors and imaging agents, and a particularly effective way to develop indicator displacement assays that operate in water.
GISH, a method of modified fluorescent in situ hybridization (FISH), uses the total genomic DNA as a probe (Heslop-Harrison et al., 1988; Schwarzacher et al., 1989) and usually with an excess of unlabelled DNA from another species as blocking agent for better differentiation (Anamthawat-Jonsson et al., 1990).
These findings are different from what has been previously reported in a study using a different molecular method of analysis (fluorescent in situ hybridization), which reported, "the absence of a correlation between the bacterial species Staphylococcus aureus and Pseudomonas aeruginosa detected in wound by culture and what is in fact present" [ 5].
Using a previously established method of attaching fluorescent probes to the large and small ribosomal subunits, the transitions between the non-rotated and rotated states of individual ribosomes can be tracked as they translate along an mRNA (Marshall et al., 2008), providing translation times at each codon.
In this study, we have used alkyne azide click chemistry to develop a method for fluorescent labelling of PPMOs and exemplified this using a Cy5 fluorophore.
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