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Figure 3 illustrates the procedure of fluorescent cell searching based on K-mean clustering and PCA.
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In comparison to prior studies, this developed strategy here not only simplifies the preparation procedure of the fluorescent probes but also can be applied in sensitive determination of Hep with good accuracy.
Spectrofluorometry using an optical fiber is a non-invasive procedure for the detection of fluorescent tracers.
This approach gave results comparable with the epifluorescence microscopy procedure of measuring accumulation of fluorescent dye in living mussel gills (Cornwall et al. 1995; Eufemia and Epel 1998; Galgani et al. 1996) but was less susceptible to subjective errors connected with the microscopic assay, such as the selection of a tissue area to be measured.
Here we describe procedures for the conversion of fluorescent differential display results into quantitative transcription profiles, using a DNA sequencer for cDNA fragment separation and DNA fingerprinting software for band matching, data normalization and densitometry.
The mutational status of CALR was determined at diagnosis using previously described procedure of high-resolution capillary electrophoresis of fluorescent dye-labeled PCR amplicon of CALR exon 9; samples with abnormal peaks were subjected to conventional bidirectional Sanger sequencing.
With the upcoming field of image-guided surgery, not only traditional 99mTc- and 111In-labelled radioguidance procedures blossom, but also the development of fluorescent and hybrid/bimodal tracers gains increasing interest.
The technique for incorporation of water-soluble and stabilized with three-functional polyethylene glycol derivatives core/shell QDs into the polymer wall of the microcapsules and detailed characterization at each stage of experimental procedure ensured efficient fluorescent encoding of microcapsules.
Procedure for DNase treatment of samples, construction of fluorescent cDNA, and hybridization to arrays were carried out as previously described with minor modifications [36].
(C ) Schematic representation of the procedure used to quantify the nuclear translocation of fluorescent protein fusions into intact nuclei.
The sensitivity and resolution of the in situ hybridization procedure has been enhanced with the use of fluorescent dendrimers as probes for mRNA.
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