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We derive this protein interaction sub-network using a nearest-neighbor expansion method initially described in [ 27].
After antigen retrieval, the slides underwent a protocol used to identify the appropriate antigens, modified from the ABC method initially described by Hsu et al (1981a, 1981b), following the protocol available from Vectorlabs (Vectorlabs, UK).
The only fully automated method of plasma antioxidant activity in the market is the Total Antioxidant Status assay by Randox (Antrtim, UK), based on the TEAC method, initially described by Miller and Rice-Evans [ 21- 23].
After 10 minutes, the positions of the limbs were rated three times on a numeric scale during a 15-minute period, according to the method initially described by Attal and colleagues [ 20] and used by our group [ 21].
Determination of the sun protection factor (SPF) was done by the spectrophotometric method initially described by Diffey and Robson, 9 then modified and improved, in view of the evaluation of the protection of the product against effects of ultraviolet B and ultraviolet A on the skin.
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The method is initially described in a geometrical sense, then presented in a practical implementation using existing vision and registration algorithms.
The method was initially described for proteins expressed in 96-well plates, with small-scale purification in a 96-well format and detection of proteins blotted onto filters using antibodies to the purification tag [ 28 ].
We used a method derived from that initially described by Nakamura et al (2002).
The SAGE method was used as initially described in Velculescu et al. [ 81, 82] at the CHUQ Research Centre (CRCHUQ) SAGE Platform, Quebec City, QC, Canada http://www.crchuq.ulaval.ca/plateformes/gpb.
Detection of the GSTM1 and GSTT1 null alleles was performed with a method modified from that initially described by Arand et al. (1996).
Recursive partitioning analysis (RPA), a method of classification, was initially described by the Radiation Therapy Oncology Group and was intended to provide a way that divide patients into homogenous groups based on the length of survival [ 3].
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