Sentence examples for method essentially described from inspiring English sources

CD22-expressing cells were isolated by fluorescence activated cell sorting (FACS) after staining with a mouse anti-human CD22 monoclonal antibody directly conjugated to phycoerythrin (anti-human CD22RPE, Clone 4KB128, Dako, Golstrup, Denmark), by a method essentially described previously [ 25].

Reading your thoughts (IEEEEE!!!) is a hyperbolic internet-age method of essentially describing a wire-tap.

Total RNA was extracted using the hot acid-phenol method essentially as described [15].

N2aβ and 293T cells were transfected using the calcium phosphate method essentially as described [58].

For transient transfection, 293 cells were seeded in DMEM supplemented with 5% charcoal dextran-treated FBS, and plasmid constructs were introduced into cells using the calcium phosphate precipitation method essentially as described [65].

NAA (µmol/L) was measured using a modified stable isotope dilution Gas Chromatography-Mass Spectrometry (GC-MS) method essentially as described by Jakobs et al. [7] In short, 1 nmol [D3]-NAA was added as internal standard to 100 µl of CF. This was also added to aqueous standards.

Quantification of mtDNA was conducted using a rt-qPCR method essentially as described [24].

Drug sensitivity was determined by the MTT method essentially as described by the manufacturer's instructions (Sigma, St . Louis MO, USA).

Na+ and K+ content in plant tissues was determined by the freeze-thaw method essentially as described elsewhere [ 54].

The presence of FCN3 gene 1637delC polymorphism was investigated by a PCR RFLP method, essentially as described previously [ 14].

Interacting proteins were eluted with 6M urea, and samples were prepared for analysis using a filter-aided sample preparation method, essentially as described (Wisniewski et al., 2009 ).