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Application of this method in populations with wide availability of HIV treatment (ART) may require identifying people on ART as a separate group.

The transferability of this method in populations that have known differences in LD structure, like African, Japanese, Chinese, still needs to be determined.

Another limitation is that the population studied was almost entirely composed of patients with malignant neoplasias and BMI < 30 kg/m2 and the validity of the method in populations with neurological diseases and different BMI profiles needs to be evaluated.

The MAF distribution in RRL method, Illumina 1 M chip and HapMap populations were plotted, demonstrating the real MAF distribution of RRL method in populations without any bias (Additional file 2).

33 The use of HbA1c to diagnose T2D may also be a less reliable method in populations in which T2D is best diagnosed by 2 hour plasma glucose than by FPG.

Moreover, strong bottleneck signatures may not be detected by this method in populations that fluctuate in size where Ne is initially low and reductions in census size do not generate strong reductions in Ne [ 13], as is likely to be the case in our tested populations.

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In applications of the capture-and-remove method in population biology the number of observation occasions (captures) might often be determined by the design of the study.

For our primary analyses, we used the definition of Cella et al (2002b), which was developed to combine anchor- and distribution-based methods in populations similar to those we studied.

Moreover, looking at a potential HZ incidence trend overtime is challenging and depends on the availability of baseline data collected using comparable study methods in populations with comparable health care behavior.

They are almost the only method useful in populations with poor literacy.

Allelic richness was estimated using the rarefaction method implemented in populations 1.2.30 (Langella 1999), which estimated the mean number of alleles per locus for a reduced sample size.

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