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Although many species can reproduce asexually and use similar mechanisms to regenerate after severe injuries, sexual reproduction is the normal method in species whose reproduction has been studied.
By assessing the bias introduced to estimates of N e by the use of the LD method in species with overlapping generations, our results can inform sampling designs in future studies that seek to estimate one of the most important population parameters in evolutionary and conservation biology.
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Here, we provide the first detailed assessment of intra- and inter-observer consistency of the Mackinnon List method in generating species abundance indices that could be useful for conservation monitoring purposes.
We used the ratio of the length of the visible genital segment 8 to segment 6 (S8/S6; Figure 1) as an approximate index of the proportion of the genital segment length that is not shielded within segment 7 (see Methods and Figure S2 for validation of this method in three species of Gerridae).
Wiens [ 25] described a "wish list" for the ideal method in delimiting species.
Additionally, all cervid samples did label with antibody P4 in the absence of proteinase K treatment indicating that PrPC is detected by this method in all species examined (data not shown).
Also, the effectiveness of the MulRF method in inferring species trees from multi-copy gene trees suggests that other tree distance measures can be used in the same context.
Most plant miRNA families have been identified by traditional Sanger sequencing method in model species with known genome sequences (Arabidopsis, rice and poplar) and most miRNAs are conserved across plant families [ 12].
It has rapidly become a popular method in aquatic species for quick SNP discovery and genotyping for building linkage maps [ 34], population genomics [ 35, 36], comparative genomics [ 37] and QTL mapping [ 38, 39].
To apply our method in different species, one would need to create a new Sanger validated dataset to account for its particular genomic properties such as the degree of heterozygosity or repetitiveness.
Genotyping by sequencing (GBS), using methylation-sensitive restriction enzymes to reduce genome complexity while retaining good genome coverage of lower copy regions, has proven to be a highly efficient method in different species [ 20– 25].
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