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Here, we describe an automated high-throughput NMR-based metabolomics method for urine analysis.
The CE-MS method for urine proteomic analysis has high sensitivity and reproducibility [ 38].
In conclusion, the mSPE method was found to be superior to the conventional, standard SPE method for urine peptide sample preparation when applying LC-MS peptidomics analysis due to the optimized sample clean up that provided improved experimental inference from the confidently identified peptides.
For proteinuria, when the analysis was limited to studies that used filtration only as the concentration method for urine microscopy, sensitivity was comparable (62%52%to71% 71%) vs 61% (53% to 69%) and specificity was lower (80%73%to86o 86%) than those produced by the overall analysis (83% (77% to 88%)) (Table 1; Table 2; Table 3).
For microhaematuria, when the analysis was limited to studies that used filtration only as the concentration method for urine microscopy, sensitivity (73%69%to78% 78%) vs 76%72%to80% 80%)) was lower and specificity was comparable (86% (82% to 89%) vs 86% (82%to8989%)) with those produced by the overall analysis.
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As a result, it was essential to examine methods for urine concentration.
Further investigations of extraction methods for urine will need to be undertaken to monitor the impact of inhibitors.
The methods for urine and blood specimen analysis at the Yaoundé Central Hospital's laboratory have been described in details elsewhere [ 20].
We therefore recommend the standardisation of methods for urine testing to minimise variation before incorporating urinary detection of HPV into guidelines for cervical cancer screening.
Methods for urine dilution adjustment (unadjusted, creatinine adjusted, creatinine z-score adjusted) did not meaningfully change our reported measures of variability (Table 1).
The Fisher exact test was used to evaluate whether clinically relevant bacteriuria detected by the reference standard was influenced by the method used for urine collection or by the time elapsed between sample collection and laboratory analysis.
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