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One microliter sample was injected in a split mode (10 1) for PGS and 5 μl sample at a splitless mode for urine.
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ANOVA analysis for urine Na: p = 0.04.
ANOVA analysis for urine output: p = 0.048.
Urine was measured for urine volume and radioactivity concentration as measured in a calibrated gamma counter.
For urine collection, consent was sought from the male students.
Protocols for urine collection and sample processing have been developed by European Kidney and Urine Proteomics (EuroKUP) and Human Kidney and Urine Proteome Project (HKUPP) (http://www.eurokup.org/node/138) [11].
No further pre-treatment was required for urine samples.
A bladder catheter was placed for urine collection.
However, urine is a highly variable sample matrix that may exert matrix effects on assays for urine albumin.
Accounting for urine dilution.
Check clothing for urine.
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