Ai Feedback
Exact(4)
The screening protocol entails a new and fast method for mutant strain selection altered in their oxygen production/consumption balance.
The new protocol introduced a novel Tc-PCR method for mutant screening which can more efficiently and accurately select against parental molecules and undesired mutations in mutagenic sequence segments.
Furthermore, the proposed method allows one to skip screening steps because it uses DNA pooling and a commercial dual indexing system, which enables simple and effective detection of plants with functional mutation and can be used as an alternative method for mutant screening.
High resolution melting (HRM) analysis [ 23, 24] has been recently applied as an alternative simple, rapid, and inexpensive method for mutant discovery in tomato [ 25] and wheat [ 26], although its accuracy depends on the quality of PCR, resolution of the instruments, and the choice of fluorescent dyes [ 24].
Similar(56)
Normal PCR thermal cycling instruments can well perform the method for mutant-enrich PCR.
We developed a new method for mutant-enrich PCR only with snapback primer (SPACE-PCR), in which the enrichment efficiency is nearly 100-fold.
Sophisticated methods for mutant isolation and mutational mapping by recombination have been described for a few prototype phages such as λ, T4, and T7, and although in principle these could be applied to other phages, there are few examples of this.
The success in expressing the mature cytochrome c″ in E. coli allows the engineering of the cycA gene by site-directed mutagenesis thereby providing an ideal method for producing mutant protein for studying the structure/function relationship.
Thus, we suggest that, to further promote the clinical applications of bioactive proteins, it is necessary for the protein-drug innovation system to combine 1) a method for creating mutant proteins (muteins) with desired properties and 2) a bioconjugation method that generates conjugated products with optimal properties, such as targeting capability.
A popular alternative method for predicting mutant behavior is based on the Minimization of Metabolic Adjustment (MOMA) [ 15].
We identified a method for comparing mutant frequencies among robust and brittle strains of virus φ6 which should be less confounded by any of their underlying differences in genetic architecture in gene P3.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com