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The method for apoptotic cell counts in embryos of diabetic mice as described previously [ 34].
Our method combines the advantages of the 96-well format and the conventional EB/AO method for apoptotic quantification.
This modified method combines the advantage of the 96-well format and the conventional EB/AO staining method for apoptotic quantification.
In this study, we have used the terminal deoxynucleotidyl transferase biotin-dUTP nick end labelling (TUNEL) method for apoptotic cell detection (Gavrieli et al, 1992) in post-HRMAS tissue and compared the estimated metabolite concentrations with the number of TUNEL-positive nuclei per mm in each biopsy across different grades of glioma.
To investigate the mechanism of Ad.IL3-gene-induced cytotoxicity of leukemia cells, HL60 and KG-1 were treated with PBS, Ad.IL3, Ad.IL3-PPA or Ad.IL3-MnSOD, followed by staining with Annexin V-FITC and propidium iodide (PI), a common method for apoptotic cell detection, and analyzed under a flow cytometer.
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AO/EB staining is considered an ideal method for distinguishing apoptotic cells from necrotic ones [ 29, 30].
Annexin V and PI double staining is a specific, sensitive, and quantitative method for analyzing apoptotic cells.
Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is a well-defined method for detecting apoptotic DNA fragmentation in a cell [ 32].
The use of the TUNEL assay and agarose-gel electrophoresis to assess DNA fragmentation as reliable methods for detecting apoptotic myocytes in human samples contributed to the knowledge about the role of cell death in heart failure but unwittingly biased the interpretation of the mechanisms involved in this event [37], [38] and others.
One of the greatest technical challenges facing investigators in this field of study is the requirement for accurate, consistent, and convenient methods for identifying apoptotic chondrocytes in cartilage.
A common method for detection of apoptotic cells is based on staining with Annexin-V and a DNA-binding dye that does not penetrate intact cell membrane (e.g. PI).
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