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A representative picture of the caspase-3-positive staining used for apoptotic cell counts is shown in Figure 4.
In addition, some monocyte samples were also used for apoptotic cell clearance assays (see below).
Samples used for apoptotic studies.
EzWay Annexin V-FITC apoptosis detection kit (Komabiotech, Inc, Seoul, Korea) was used for apoptotic analysis.
Twenty intact crypts were used for apoptotic cell identification per animal.
A kit for fragmented DNA detection (US1QIA33-1EA; TdT FragEL TM DNA Fragment. Detect, Merck) was used for apoptotic nuclei detection.
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The TUNEL assay is widely used for detecting apoptotic cells.
A cell death detection ELISA plus kit (Boehringer Mannheim, Indianapolis, IN, USA) was used for assessing apoptotic activity by detecting fragmented DNA within the nucleus in ABT-737-, candstol-, and combination of ABT-737 and cafestol-treated cells.
Figure 6b shows that VIP receptors on acinar cells are functional because 100 nM VIP stimulated cAMP accumulation and amylase secretion at the same concentrations used for inhibiting apoptotic signals.
The DeadEnd™ Fluorometric TUNEL System (Promega) was used for detection of apoptotic cells.
Well-known morphological criteria were used for identification of apoptotic and necrotic cells.
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