Sentence examples for methanol for one from inspiring English sources

MCF7-ERβ cells were plated on cover slides at approximately 50% confluence and fixed with cold methanol for one hour followed by permeabilization with 0.1% Triton X-100 for five minutes on ice.

The skins remained in methanol for one hour to inactivate the glucosidase enzymes [ 36].

Samples were fixed by washing polyps three times with ice-cold 100% methanol for one hour at 4°C.

After incubation for two hours at 37°C, the membranes were removed, fixed in methanol for one minute, and stained with Diff-Quick (VWR International, West Chester, PA, USA).

After induction with methanol for one day, quantitative real-time PCR revealed highest mRNA expression for GST-furS-GrB, with MBP-furS-GrB and GrB mRNA levels reaching 91% and 73% of that (Fig. 3B).

The PBMC were fixed for 30 minutes in methanol and for one minute in acetone before they were washed for 3x10 minutes in a phosphate-buffered saline with foetal calf serum.

Briefly, 5 g of ground sample was extracted with 50 mL of 90% methanol in water for one hour at room temperature.

Purified enzyme having maximum cellulase activity was incubated with 30% (v/v) of different organic solvent viz., n-dodecane, n-decane, iso-octane, xylene, n-hexane, n-butanol, cyclohexane, acetone, toluene, benzene, ethanol, methanol and propanol for one week in screw crapped tubes at 65°C and 120 rpm.

Frozen tissue sections of the tumors were fixed in a cold solution of methanol:acetic acid (3:1) for one hour.

One-hundred grams of dry samples were macerated and kept in 1 L methanol at room temperature for one week, with occasional stirring.

Sypro Ruby-stained gels were washed twice in 10% methanol and 7% acetic acid for one hour each and imaged on a Typhoon 8600 imager (Amersham Pharmacia Biotech, Piscataway, NJ, USA) with 532 nm laser wavelength.