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Ultraviolet (UV) spectra of UV active compounds were recorded in methanol by using a Shimadzu UV-240 and U-3200 Hitachi spectrophotometer.

The quantum yields of methanol by using GO-Cs2Mo6Bri8Brax and GO- TBA)2Mo6Bri8Brax as catalysts with reference to Mo6 cluster units presented in 0.1 GO- TBAt of catalyst were found to be 0.015 and 0.011, respectively.

The coarse leave samples were extracted with methanol by using a Soxhlet method and the obtained methanol extract was defatted and fractionated by different polarity of solvents with increasing polarity to give hexane, chloroform, ethyl acetate, butanol, and water extracts, respectively.

The temperature was calibrated with 100% methanol by using chemical shift differences of methyl and hydroxyl protons.

Tumors were weighed, cut into pieces, and homogenized in 5-ml methanol by using a Polytron and extracted with methanol and 3 times with ether/CH2Cl2 (5:1).

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Powdered material (1.2 Kg) was extracted successively with petroleum ether (PET), acetone (ACE) and methanol (MeOH) by using Soxhlet apparatus.

For all performed experiments, the feasibility of the separation has been proved and the possibility to separate mixtures with high methanol concentration by using a membrane distillation based micro-separator has been for the first time reported.

The analysis revealed that five peptide sequences have similarity with the stretches of secreted lipoprotein of Methylomicrobium album BG8 (Protein View: gi|381152247) and displayed 70% coverage with a significant homology (Figure 5). Figure 3 Separation of the antimicrobial lipopeptide from acidic methanol extract by using reversed-phase HPLC.

Moreover, Rajeh et al. [ 13] also reported the cytotoxicity activity of E. hirta leaves, stems, flowers, and roots methanol extracts by using brine shrimp lethality assay.

The air-dried and powdered plant material (50 g) was extracted with 500 ml methanol (CH3OH) by using a Soxhlet apparatus for 8 hours.

The radical scavenging activity was calculated from the equation: % of radical scavenging activity = Abs control - Abs sample/Abs control × 100 The screening of chemical constituents was carried out with the methanol extracts by using chemical methods and thin-layer chromatography (TLC) using different mixtures of organic solvents as mobile phases.

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