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Following infection with Trx-CFP rAAV, the cells were harvested by trypsinization, washed twice with sterile calcium/magnesium-free PBS, re-suspended in cold PBS at ∼2×106 cells/ml and filtered through a 50 µM mesh to generate a single-cell suspension.

Spleens from C57BL6 mice were dispersed through nylon mesh to generate a single-cell suspension.

Enzymatically dissociated tumour was then filtered through 150 μm nylon mesh to generate a single cell suspension.

The suspension was filtered through a 70-μm stainless steel wire mesh to generate a single-cell suspension.

Stimulated cells were then trypsinized, strained through a 40 μM mesh to generate a single cell suspension at ~5x10 cells, and then sorted on a FACS Vantage with DiVa (Digital Vantage) upgrade (Becton Dickinson).

Similar(55)

The inspection is completed using two commercially available quadcopters, in a laboratory environment, with the acquired visual inspection data processed and photogrammetrically meshed to generate a three-dimensional surface-meshed model of the drum.

Moving mesh partial differential equations (MMPDEs) are used in the MMPDE moving mesh method to generate adaptive moving meshes for the numerical solution of time dependent problems.

We configure the mesh algorithm to generate a denser mesh near the top of the head or close to the complex cortex surface.

We used a combination of keywords and medical subject heading (MeSH) terms to generate 3 subsets of citations: 1 relating to exposure, 1 on indexing outcomes, and 1 for study designs.

Many classical numerical schemes rely on the application of strict meshing structures to generate accurate solutions, which in some applications are an infeasible constraint.

Phase and correlation maps were converted from individual surface space to the standard-mesh surface to generate group average data.

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