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Cytoskeleton elements are clearly visible underneath the plasma membrane of control cells.
Additionally, ROBO1 is located at the outer membrane of control, but not ZEB1 knockdown cells (Supporting Information Fig S3A).
Note that, as awaited, Cut-positive cells are never present in the peripodial membrane of control discs (Supplementary Figure 5).
Immunolabeling with antibodies against L1 and caveolin showed a homogeneous pattern in the plasma membrane of control and EtOH-treated 2A2-L1s cells.
Antibodies against PDX1 and glucose transporter-2 (GLUT2) stained strongly the nuclei and cell membrane of Control islets, but weakly and very faintly of pBmpr1aHet and pBmpr1aKO islets, respectively (Fig. 2C).
In all cases, the reduction in plakoglobin expression was translated into reduced γ-catenin levels, with high levels of γ-catenin detected on the cell membrane of control MCF7 and T47D cells and reduced staining detected on the cell membranes of plakoglobin-knockdown cell lines (T47D cells shown in Figure 1E).
Similar(53)
A high expression level of EGFR was observed in the tumor cell membranes of control mice.
Similar levels and distribution of SIMU and DRPR were observed on glial membranes of control and mutant embryos.
In comparison, membranes of control cells, which did not contain filipin in the fixative did not display deformations.
Even Golgi membranes evident in tissue sections and in isolated MEF cells were indistinguishable from the stacked and linked Golgi membranes of control samples.
Interestingly, prolonged incubation in serum free medium, increased the binding of [H]NMS to membranes of control cells incubated to 7 or 15 days compared to cells incubated for 3 days in the absence of FBS.
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