Sentence examples for membrane inserts were from inspiring English sources

The membrane inserts were placed into six well culture plates and 1.4 ml of R16 medium with supplements [47] was added.

Slice cultures on membrane inserts were transferred to a submersion-type recording chamber continually perfused with 30 32°C oxygenated (95% O2 5% CO2) artificial cerebrospinal fluid (aCSF) solution containing (in mM): 117 NaCl, 4.7 KCl, 2.5 CaCl2, 1.2 MgCl25 25 NaHCO3, 1.2 NandPO4 and 11 glucose.

Polycarbonate membrane inserts were used for transport studies and polyester inserts were used for imaging, all with 3.0 μm pore sizes.

From ALI cultures, membrane inserts were washed twice with PBS and fixed with 4% paraformaldehyde in PBS (Histofix; Carl Roth GmbH, Karlsruhe, Germany) at 4°C for 2 h or overnight.

Cytocentrifuged fresh cells and confluent EBECs cultured on glass cover slips or membrane inserts were washed twice with PBS after medium removal, and fixed in ice-cold acetone or methanol for 5-10 min at -20°C (acetone fixation used for all CK and VIM detection, methanol for ZO-1 detection).

The morphology of 2B4 cells grown in the membrane inserts was subsequently examined by TEM.

The 5 µM thin sections of the epithelial cell monolayer on the membrane insert were stained with H&E and periodic acid Schiff (PAS -stain.

Then, the invading cells at the bottom of the membrane insert were detached, and the invasive potential of the cells was evaluated microscopically by counting the number of invading cells.

After the cells were incubated for 24 h at 37°C in a humidified incubator with 5% CO2, The invasive cells attached to the lower surface of the membrane insert were fixed in 10% formalin at room temperature for 5 min and stained with 0.05% crystal violet.

The invasive cells on the lower surface of the membrane insert were fixed with 4%% paraformaldehyde for 30 min, permeabilized with 0.2 % Triton X-100 at room temperature for 15 min, and then stained with 0.1 % crystal violet for 5 min.

The top side of the membrane insert was seeded with eGFP expressing NIH 3T3 cells containing nanodisks in FBS depleted medium (containing 0.1 vol% FBS).