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Finally the reuse of spent medium with proper replenishment of nutrients (nitrate, phosphate, chromium, selenium and copper) caused an unexpected 30% drop in the growth rate and did not seem to provide an attractive response as the total cost per 0.5 kg dry cell was only saved by 8 US$ a year.
For plate dilution assays, each strain was cultured overnight in synthetic dextrose medium with proper amino acid selection.
For de novo Q6 labeling experiments, yeast were grown to log phase in SD medium with proper selection and then seeded at 0.07 OD/ml into DOD medium with proper amino acid selection supplemented with 8 μg/ml C6-pABA or C6-4HB (CambrIsotopestopes).
BAC or BIBAC clones arrayed in 384-well microplates were inoculated into 96-deep well plates containing 1.0 ml TB (Terrific Broth) medium with proper antibiotics using a 96-pin replicator (BOEKEL, Feasterville, PA, USA).
E. coli transformants and DSP-10 cells were grown separately in LB medium with proper antibiotics overnight, washed in fresh medium, and mixed in a 1 3 ratio (donor: recipient) by spotting onto 0.2 μm Millipore membrane disks.
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For E. coli, the corresponding plasmids were transformed into BL21 (DE3) or TL101 competent cells with suitable antibiotics, respectively, and several single colonies were pre-cultivated in a 10 mL LB medium in 50-mL flasks with proper antibiotics at 30 °C for 12 h.
These soils are formed from volcanic material and, with proper management, have medium to high potential for rain-fed agriculture.
Fungal strains of S. pombe were grown up in YES medium (0.5% yeast extract, 3% dextrose with proper supplements) at 32°C.
With proper management, they are of medium agricultural potential.
While the differentiation pattern with proper neurite extensions was apparent in KR medium by 7 8 days post-plating (dpp) (d2+5/6), a comparable status was achieved in KO medium 1 2 days later (Fig. 1B E).
Wooden brushes with proper bristles.
Related(18)
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