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The latter is modeled as a porous medium with appropriate closure laws.
To form the bottom layer, 15 ml of each medium with appropriate antibiotics were poured onto separate plates.
ES cells were found to be able to differentiate into BE cells when cultured using medium with appropriate cell growth factors.
All strains were maintained as frozen glycerol stocks, and were sub-cultured into Luria Bertani medium with appropriate antibiotics (50 µg mL−1 kanamycin, 100 µg mL−1 ampicillin).
Furthermore, the maximum lutein productivity of 3.45 ± 0.07 mg L− 1 d− 1 was obtained upon cultivation of C. minutissima in a 2-L airlift photobioreactor using the optimized medium with appropriate process conditions.
Strains were grown on minimal solid or liquid medium with appropriate supplements (simplified as MM; [39]).
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Recombinant proteins were precultured overnight at 37°C in LB medium supplemented with appropriate antibiotics.
Yeast strains were cultivated at 30 °C in SC medium supplemented with appropriate nutrients.
For RNA isolation, yeast cells cultivated on SC medium supplemented with appropriate nutrients were collected when OD600 reached 1.0.
Chromosomal DNAs from S. cerevisiae cultured in SC medium supplemented with appropriate nutrients were embedded in agarose plugs as described by Sheehan and Weiss (Sheehan and Weiss 1990).
Transformants obtained using the lithium-acetate method (Chen et al. 1992) were selected on SD medium supplemented with appropriate amino acids and nucleotides.
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