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Exact(5)
On day 82, a 37% higher SMA on methanol was achieved in a medium with complete metal solution compared to a medium with complete metal solution except nickel.
After 24 hours, cells were washed and fresh medium (with complete serum) was added.
Remaining extracellular bacteria were then killed by replacement of the medium with complete medium containing 30 μg ml−1 gentamicin.
The transfected cells were seeded on Transwell chambers (Corning Life Sciences, Corning, NY, USA) before replacing the transfection medium with complete medium with or without Dex.
The best-performing strain, GS1.11-26, showed a maximum specific D-xylose consumption rate of 1.1 g/g DW/h in synthetic medium, with complete attenuation of 35 g/L D-xylose in about 17 h.
Similar(55)
At 5 hrs, the medium was replaced with complete medium.
After 6 h of incubation, the medium was replaced with complete medium.
After 1 h incubation, medium was replaced with complete medium containing 0.75% agar.
After 8 h, the medium was replaced with complete medium.
After 5 hours the medium was replaced with complete medium.
Then the medium was replaced with complete medium.
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