Sentence examples for media with complete from inspiring English sources

However, the NE-like phenotype is reversible upon replenishing the media with complete serum during several weeks [20].

We have also observed restoration of acid-fastness, when the dormant cells generated under multiple-stress were grown in fresh media with complete nutrients (data not shown).

Consistent with an hypothesis of an adaptive functionality for SphK1, replenishing the media with complete serum was associated with the reversal of SphK1 activity towards its basal levels.

Interestingly, the reversability induced by replenishing the media with complete serum for 21 days was associated with a profound down-regulation of SphK1 activity (Fig. 9C), which went back to the levels found in parental cells.

Y2H, yeast two-hybrid; PCI, phenol-chloroform-isopropanol (25:24:1); YPD, yeast-peptone media with dextrose (2%); SD, synthetic defined media with complete supplements except as indicated; CIP, calf intestinal phosphatase.

Cells were spun at 30°C for 1 hr at 2,400 rpm, allowed to recover for 4 hrs (37°C, 5% CO2), spun again for an additional 1 hr, and then the media replaced with complete RPMI-1640 growth media.

For the NHY942/NHY943 background strains, tetrads were dissected and germinated on yeast extract-peptone, 2% dextrose media supplemented with complete amino acids.

Within 24 hours following the transfection the media was replaced with complete media.

The media was replaced with complete media (2 4 ml) after 5 h incubation at 37°C with the ASOs.

Chromatin complexes were cross-linked by replacing the culture media with fresh complete media containing 1% formaldehyde, and cells were incubated at room temperature for 10 min. Cross-linking was quenched by adding glycine to a final concentration of 0.125 M. Cells were washed two times with ice-cold PBS, and cell pellets were flash-frozen in a slurry of dry ice and ethanol.

After 24 h, the media was replaced with complete growth media (100 μL/well).