Sentence examples for medium were gradually from inspiring English sources

Cells cultured in 2% FBS medium were gradually weaned to reduced serum (0.5% FBS) prior to use in invasion assays since immediate serum withdrawal resulted in massive cell death even when cells had been routinely cultured in medium containing 2% FBS.

Serum concentration in medium was gradually increased to 10% and 20%.

After 10 passages, the percentage of FBS in the medium was gradually reduced.

At 50% confluence, the medium was gradually changed to neural basal medium (Invitrogen) containing N2 and B27 (Invitrogen).

Subculture was repeated three times and zeatin concentration in the medium was gradually decreased (1.5, 1.0, and then 0.75 mg/l).

Cells were allowed to adhere to the scaffold for 2 h during which culture medium was gradually added every 30 min.

From D4, in the presence of SB/DM, the medium was gradually replaced with N2M (DMEM-F12; 1 × N2 Supplement, Life Technologies-Thermo Fisher Scientific; 1 × NEAA, Life Technologies-Thermo Fisher Scientific; 2  μg/ml heparin, Sigma-Aldrich).

The oxygen dissolved in the culture medium was gradually depleted by bacteria, as demonstrated by decolorization of the oxygen-sensitive probe methylene blue.