Sentence examples for medium was gradually from inspiring English sources

Serum concentration in medium was gradually increased to 10% and 20%.

After 10 passages, the percentage of FBS in the medium was gradually reduced.

At 50% confluence, the medium was gradually changed to neural basal medium (Invitrogen) containing N2 and B27 (Invitrogen).

Subculture was repeated three times and zeatin concentration in the medium was gradually decreased (1.5, 1.0, and then 0.75 mg/l).

Cells were allowed to adhere to the scaffold for 2 h during which culture medium was gradually added every 30 min.

The oxygen dissolved in the culture medium was gradually depleted by bacteria, as demonstrated by decolorization of the oxygen-sensitive probe methylene blue.

Cells cultured in 2% FBS medium were gradually weaned to reduced serum (0.5% FBS) prior to use in invasion assays since immediate serum withdrawal resulted in massive cell death even when cells had been routinely cultured in medium containing 2% FBS.

At higher concentrations of phosphate in the medium, selenium transport was gradually replaced by low-affinity transporters (Pho87p, Pho90p, and Pho91p).

The pH value of the fermentation medium was decreased gradually to 6.0, and then maintained by the automatic addition of 5 M NaOH.

The initial concentration of sucrose in the medium was 100 g/L and it was gradually consumed during the course of fermentation and lead to curdlan production.

Peripheral blood mononuclear cells isolated from HLA-A*0201 HLA-A*0201ealthy donors or positive were stimulated withealthyen by autologous presentation of peptidonorsuted at a final corcentration of 10–6 or 10–7 M. Cells were maintained in R10 medium to which IL-2 (patientsh) weregradually added from day 3 postimulatedtion up to a maximum concentration of 100 U/mL.