Sentence examples for medium washed with from inspiring English sources

To evaluate if PrPC or mutant PrP forms influence cellular iron uptake, M17, PrPC, PrPΔ51 89, PrPΔ23 89, and PrP102L cells cultured in serum-free medium for 1 hour were radiolabeled with 59FeCl3-citrate complex for 4 hours in the same medium, washed with PBS supplemented with 100 µM desferrioxamine (DFO) to remove surface bound iron, and lysed in non-denaturing buffer.

The MSCs were dissociated from the culture dishes with 0.25% trypsin, neutralized with culture medium, washed with PBS and collected by centrifugation at 900 × g for 5 min at room temperature.

Briefly, cells were splitted and seeded into 12-well plates and, after aspirating the culture medium, washed with 1 ml of Hank's balanced salt solution (HBSS) (Life technologies S.r.l) supplemented with Hepes 10 mM, pH 7.3.

For flow cytometry experiments, cells were trypsinized on the plate, resuspended in the culture medium, washed with PBS and stained with Annexin V-PE using the Apoptosis Detection Kit (BioVision).

To prepare conditioned media (CM), each EOC cell line was cultured in a 100 mm diameter culture dish for 2 days in OSE complete medium, washed with PBS and incubated in serum-free medium for 2 days.

Asynchronously growing cultures were harvested by trypsinization, combined with the cells floating in the medium, washed with PBS, resuspended in 100 μl of 0.9% NaCl, squeezed through a 23.5 gauge needle into 0.9 ml of methanol and fixed for 30 min at -20°C.

After 5 7 days, cells were passaged by aspiration of medium, washing with PBS, and incubation with PBS for 5 min at 37°C.

For BaF3 cells remove medium, wash with PBS and add RPMI (with out WeHi-3b).

Cells were cultured undisturbed for a further 7 days before removal of medium, washing with PBS and fixation with 70% EtOH.

Ten days after seeding, cells were fixed by removing medium, washing with PBS and adding 4% paraformaldehyde in PBS at room temperature for 30 min.

The contribution of this basal catabolic activity could be minimized by culturing for 24 hours before aspiration of the culture medium, washing with PBS, and adding fresh culture medium [ 37, 38].