Sentence examples for medium the next from inspiring English sources

The medium was exchanged for a serum-free medium the next day and the culture was stabilized for additional 24 h.

The medium was replaced with fresh medium the next day.

Membranes were coated with 1∶25 Matrigel (Becton Dickinson) in DMEM medium and rehydrated with DMEM medium the next day for 2 hours at 37°C under 95% humidity and 5% CO2.

The normal DMEM medium was replaced with phenol red free RPMI 1640 medium the next day and incubated with LysoTracker Red- DND99 (50 nM) for 1 h to label lysosomes.

The DNA liposome-containing medium was replaced with growth medium the next morning and cells were cultured for an additional day before extraction of protein at approximately 42 hours after transfection (longer incubation times after transfection led to significant cell death that was specific to PLA2G6 plasmids).

Batch cultures were diluted to OD600nm = 0.2 into YEPD medium the next day.

When the cell culture was set up, the culture medium was replaced by a fresh medium on the next day to remove the remaining red blood cells.

Cells were plated overnight in complete medium, then this medium was replaced with serum- or glucose-free medium for the next 4 h.

Cells were washed twice with serum-free medium on the next day and further incubated in serum-free medium for 24 h.

Three electroporation reactions were reseeded in one well of a 12-well plate in growth medium without antibiotics, which was replaced by normal growth medium on the next day for RNA, protein and glycerol measurements.

After the first culture reached the stationary phase of growth on YPD medium, 1 % culture was inoculated into new 100-ml YPD medium for the next cycle of growth.