Sentence examples for medium samples and from inspiring English sources

The cells were sonicated (6 s, three times) and 20 µl of cell lysate were added to 60 µl of internal standard solution with or without formic acid as for medium samples and kept on dry ice.

To examine the effects of an impaired ERAD pathway on the GlaGus protein production or accumulation, we performed Western blot analysis on medium samples and intracellular protein samples collected from scGlaGus and mcGlaGus strains.

When comparing the YPD medium samples and the YPGal medium samples, 698 RDNPs were identified using an FDR cutoff of 5%, which is equivalent to ~ 2.2% of the yeast genome.

Total protein (60 µg) was separated on a 4 15% gradient SDS PAGE gel under non-reducing conditions for whole-cell lysate, insoluble fraction and conditioned medium samples, and under reducing conditions for soluble fraction samples and then transferred to polyvinylidene fluoride membranes (Bio-Rad).

Typical major fermentation metabolites, including ethanol, lactate, and acetate, as well as those relevant to the proposed pathway, including succinate, 1-propanol, and propionate, were detected in extracellular medium samples and their titer distributions under various culture conditions are summarized in Figure  4.

The total ion chromatograph (TIC) patterns corresponding to the compounds extracted with ethyl acetate from the control (uninoculated medium sample) and degraded samples are shown in Figure 4a-c, and their peak identity is depicted in Table 1.

In the case of the medium samples, E2 and T were measured for ovary incubations, but only T was analyzed for the testis incubations (unpublished data indicate that E2 production by testis samples is generally below detection limits).

Following the addition of fresh medium, samples A2 and B2 demonstrated that a portion of the DCs remained alive, which is in agreement with our hypothesis concerning the toxicity of the supernatant.

The culture medium was replaced every 24hr and medium samples were collected and stored −20°C until further analysis.

The medium samples were centrifuged, and the radioactivity in the pellet (intact) and supernatant (degraded) was determined.

Medium samples from infected and uninfected cells were taken at different times and frozen at −20°C until used for TNF-α determination.