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Exact(6)
In contrast, clotting times exceeded 240 seconds in all conditioned media samples, and no clot formation was observed, even at 30 minutes.
One fourth (25%) of each harvested sample (see Cell transduction) was thawed, pulverized with a pestle, and then loaded into microplates along with conditioned media samples and standards.
Lipids were next stained with iodine vapor and bands corresponding to retinol (for media samples) and retinyl palmitate (for cell samples) were counted for tritium by scintillation counting.
Aliquots of media samples and cell lysates from the wells were collected on day 7 in order to allow the secreted soluble proteins to accumulate to detectable levels.
Only proteins identified in conditioned media samples and not in basal media (that is, in the baseline medium prior to culture) and exclusively identified by peptides consistent with porcine origin were kept.
Sulphated glycosaminoglycan (sGAG) content was determined using the 1, 9-dimethyl-methylene blue dye-binding assay in agarose/papain digests and media samples and the values normalized to DNA levels [ 25, 26].
Similar(54)
For controls or blanks, a second set of tubes containing liquid media sample and buffer was incubated as described above.
The fluorescence of each sample is proportional to the concentration of PRL in the media sample and was measured on a time-resolved fluorometer, VICTOR™ 1420 Multilevel Counter (Wallac, PerkinElmer LAS (UK Ltd , Beaconsfield, UK).
We found the G/D ratios of the condition media sample and control were 14.5 and 6.6, respectively, suggesting that intertube distances are greater for condition media formed aggregates.
This includes differences in DTCA data collection methodology (i.e. media sampling and channel monitoring strategies), differences in defining the pharmaceutical market/DTCA mediums, and differences in methods for calculating expenditures.
The quantities of analytes (individual flavonol conjugates or total conjugates) present in the media from 10 cm dishes and apical/basolateral media samples from transwells, and in cell fractions, were calculated from the respective HPLC chromatogram peak areas using standard curves generated with authentic standards where available or similar analytes where standards were not available.
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