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Mechanical property index Imp could well comprehensively characterize physical properties of medium, which could be useful for guiding initial culture medium preparation and SSF process control.
A biosynthesis cost is generally determined based on the costs of nutrients used for culture medium preparation and the costs of broth separation by integrated downstream processes.
Standard yeast protocols were used for strain generation, growth, medium preparation, and DNA damage sensitivity assays.
Tumor tissue dissociation and procedures for medium preparation and expansion of LCSC in vitro were performed as we previously described.
Ultra-pure water used in medium preparation and assays was processed with a Milli-Q Gradient system from EMD Millipore (ON, Canada).
To minimize differences in gene expression due to culture and sample workup conditions, all samples that underwent direct comparison of gene expression profile were cultured and harvested at the same time (3 days after passaging) using the same medium preparation and RNA isolation reagents.
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Zalis et al. compared barium and iodine contrast medium preparations and found improved discomfort scores and readability in the patient group that had ingested an iodine tagging agent [ 10].
M. Symonds provided laboratory support, collection of sera for the inhibition component of the study, growth medium preparation, culture maintenance and assistance with the writing and review of the manuscript.
Records of routine procedures carried out, such as cell counts, cell-line passaging and medium preparation can be kept on standard forms designed for the purpose.
For glucose medium preparation, 56.7 g/L of glucose and 1 g/L of yeast extract were sterilized at 121 °C for 15 min followed by cooling to room temperature.
Embryos that had progressed to at least the 2-cell stage were cultured in 20 μl drops of KSOM (Lawitts and Biggers, 1993) (prepared according to the Jackson Laboratory medium preparation protocol) covered with mineral oil (FertiPro) in a humidified chamber at 37°C supplemented with 5% CO2.
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