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"Handspring's ongoing project," said Adrian Kohler, a co-founder of the company, "was our assault on the notion that puppet theater is a medium only for children".
Four wells in each plate were not inoculated (LB medium only) for negative control references.
Control wells (cells treated with medium only) for HEC-1-A (upper panel) and Caco-2 (lower panel) cells varied by no more than 24% in their TER over the course of the 24 h period (Figure 5).
The following day, the cells were treated with human recombinant IL-1β (50 ng/ml) (PeproTech, Rocky Hills, NJ) or medium only for 24 hours.
For MTT assay, OvCa3 or A2780 cells were seeded in 96-well plates and treated with 10 or 100 n M CRH, or medium only for 24, 48 or 72 h (five wells per condition, 10 cells per well).
The LysoTracker dye was used at a very low concentration in the cell-incubating medium only for 5 minutes before cell fixation in order to stain mainly lysosomes in the cells [ 41].
Similar(51)
Approximately 90%% of the CMC activity was located in the cytoplasm (5.36 U/g DCW), while the activity in the periplasmic space and extracellular medium only accounted for 6.31%% (0.41 U/g DCW) and 5.90 % (0.38 U/g DCW) of the whole cell activity, respectively.
Compared to SCD medium, SMD medium was synthetic medium only supplemented for auxotrophic requirements.
After adhesion, cells were treated with DHPMs or maintained in culture medium only (control) for 72 h.
It was also found that the medium only accounted for the increase in ornithine, since it was the same in the presence or absence of the cells.
DNA micro-array analysis was performed using amplified RNA, purified from cryopreserved PBMC that were later thawed and incubated with BCG, PPD or medium only (unstimulated) for 12 hours.
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