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While conidiation does not normally occur under these conditions, transfer to medium lacking a carbon source does induce conidiation in submerged cultures.
For nodule induction, the growth medium was replaced with fresh medium lacking a nitrogen source three days before inoculation with Sinorhizobium meliloti strain Rm1021 pXLGD4 RCR 2011(GMI 151).
In the wild-type strain very low levels of sterigmatocystin were detected after 24 h growth in medium containing glucose and much higher levels after transfer, for 24 h, to medium lacking a carbon source.
Pexophagy was induced by transferring cells to starvation medium lacking a nitrogen source (SD-N; 0.17% yeast nitrogen base without amino acids and ammonium sulphate, 2% glucose) (Hutchins et al, 1999; Manjithaya et al, 2010a).
While the level of nitrogen that may be leached from the cells transferred to the medium lacking a nitrogen source was not determined; it is clear that the cells under this low nitrogen condition were stressed.
The transcriptional control of meiosis in budding yeast is of special interest, since it is composed of several transcriptional waves, controlled by different transcription factors, and has therefore been studied extensively [ 3] In yeast, meiosis initiates in diploid cells upon exposure to medium lacking a fermentable carbon source and nitrogen.
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DOI: http://dx.doi.org/10.7554/eLife.02409.006 Using live cell microscopy, we found that mCherry-tagged Gln1 was diffusely localized in dividing cells but formed filaments when the growth medium lacked a carbon source (33% of the cells had filaments after 4 hr of glucose starvation).
Explanted CNSs were cultured in a saline medium lacking all lipids, sugars, and amino acids.
For the Rh1-Gal4 experiment, flies were reared in a medium lacking vitamin A to accumulate Rh1 apoprotein in the ER.
To investigate whether dPob is essential for the accumulation of Rh1 apoprotein in the ER, dPob ∆4 mosaic retinas were observed in flies reared in medium lacking vitamin A, the source of the chromophore.
This makes inositol auxotrophy (the inability to grow in medium lacking inositol) an ideal screening phenotype for finding mutants with dysregulated PA signaling [ 28, 36].
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