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Transfer of C. crescentus cultures in exponential growth to media lacking a carbon source results in immediate growth arrest.
After the establishment of graft unions, successful grafts were transferred to media lacking a specific nutrient for two weeks, and miRNA abundance was analyzed in the different parts of the graft by RNA gel blots.
Cells were then washed with SMM media lacking a carbon source (SMM no carbon), suspended in anaerobic SMM no carbon (which had been kept in an anaerobic chamber for at least 4 days) to an OD600 of ~0.1 and DMSO was added to a final concentration of 0.9%.
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Yeast cells were normally grown in YP medium containing 1% yeast extract and 2% peptone or synthetic complete media lacking an appropriate amino acid for selection.
In order to randomly screen each DBD library we plated an aliquot of the DNA-binding domain library on yeast synthetic media lacking tryptophan at a density that allows the selection of individual yeast colonies.
Tandem affinity purification (Rigaut et al. 1999) with 1 l of extract grown in minimal media lacking tryptophan to an A600 ~ 2 was carried out as described previously (Mutiu et al. 2007a).
Consistent with their mesothelial nature, trypsin isolated cells cultured for 5 days in BMe (media lacking EGF) could establish a cobblestone monolayer of cells that widely immunoexpressed the mesothelial markers WT1, CD54, ZO-1 and β-catenin.
Diploids containing BD-Corolla and AD-Cona were able to grow on media lacking adenine and exhibited a deep blue color indistinguishable from the diploid positive control in the presence of X-α-gal, thus illustrating that Cona-Corolla strongly interact.
And while many Vietnamese youths simply ignore the media, those who do seek out news are faced with a print media lacking not only in teeth, but eyes, ears and any other tools they might have once used to report injustice.
For glucose deprivation, cells were re-suspended in media lacking glucose and incubated in a flask at 30°C with shaking for ten minutes.
Culture media lacking cells was used as a negative control and sodium sulfite (100 mM) was used as a positive control.
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