Sentence examples for medium incubated with from inspiring English sources

Cells were seeded into 96-well black plate at a density of 5 × 10 cells/well in 100  µL culture medium, incubated with or without experimental compounds and cultured in CO2 incubator at 37°C for 24 h.

The cells were replated at 50 to100 viable cells per cm2 in hMSC medium, incubated with a change of medium every 3 or 4 days for 7 days until reaching about 70% confluent, and lifted with trypsin/EDTA.

Cells (2.5×107) were suspended in 25 ml of culture medium, incubated with TR2J/IgG at 1 µg/ml at 4°C for 30 min, transferred to 37°C for another 1 hr, washed in phosphate-buffered saline three times, and then lysed in IP buffer for 1 hr at 4°C.

20 µl of the CellTiter 96 Aqueous One Solution Reagent (Promega, Madison, WI) were added into each well of the 96 wells plate containing cells in 100 µl culture medium, incubated with the reagent for 3 hours at 37°C in 5% CO2 and absorbance at 490 nm was recorded.

Briefly, 500000 hESCs were dissociated to single cells dissolved in 200 µl cell culture medium, incubated with virus at MOI 1 under gentle shaking for 1 h at 37°C, thereafter seeded on MEF cells in 2 ml cell culture medium as described above.

Briefly, samples of conditioned medium incubated with plasminogen were run on a 7.5 % polyacrylamide gel.

After 2 h, cells were harvested either by scraping or by removing the supernatant medium, incubating with 200 μL Non-enzymatic Cell Dissociation Solution (Sigma) and recombining with the supernatant medium.

The cells were washed once with serum-free medium and incubated with medium containing DNA, DNA-peptides corplex, or DNA complexed with cationic lipids (DOSPA/DOPE) for 8 h at 37°C.

OS cells (1.1×104) were seeded in triplicate in 96-well plates overnight in 10% FBS supplemented medium and incubated with medium only, DMSO or LLL12 at increasing concentrations for 24 hours.

The recombinant E. coli BL21 (DE3) pLysS was inoculated into 250 mL Luria-Bertani (LB) medium, incubated at 37°C with shaking at 200 rpm.

The cells were then washed twice with fresh culture medium and incubated with the new medium at 37°C for 1 h to detect the retained doxorubicin.