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After 72 h, the cells were washed with fresh medium, treated with MTT solution and incubated for an additional 3 h.
The viability of the control and the treated cells were evaluated using 3- 4,5-cimethylthiazol-2-yl -2,5-diphenyl tetrazolium bromide (MTT) assay with human breast adenocarcinoma MCF-7 cells (1 × 104/well) seeded in a 96-well microtiter plate with a 100 μL culture medium treated with various amounts of Pt NPs@alginate bubbles.
Delivery can be achieved by direct treatment of tissue surfaces or application of defined medium treated with plasma.
We examined the effect of expressing each of the five SIRT proteins in HT-22 cells maintained in normal serum medium, treated with serum-free medium or treated with HCA.
LDH activity in the medium treated with hederagenin was significantly increased (* P < 0.05, ** P < 0.01).
MiR-17- and control vector-transfected MEFs were cultured in normal medium, serum-free medium, or medium treated with H2O2.
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Colonies maintained on Matrigel and MEF-conditioned medium were treated with control medium or pertussis toxin as described above and cultured in pluripotent conditions for 24 h.
Colonies maintained on Matrigel and MEF-conditioned medium were treated with control medium or pertussis toxin, cultured in pluripotent conditions for 24 h after treatment and pulsed with 100 µM BrdU for 2 h.
Despite the findings of angiogenesis related factors in BMSC-conditioned medium, HUVECs treated with this medium showed no positive effect regarding outgrowth or organization.
To determine the percentage of intact recycled I-rhTF in the chase medium, the collected medium was treated with 15% trichloroacetic acid for 15 min at 4°C.
The following day, medium was replaced by exosome-free medium and treated with exosomes at 1X and 10X concentration, as specified.
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