Sentence examples for medium in test from inspiring English sources

The cells were then inoculated into 4 mL of fresh M9 minimal medium in test tubes containing 0 or 100 μM of cysteine to obtain an initial optical density at 600 nm (OD600) of 0.005.

After pre-cultivation and washing, yeast cells were inoculated in 5 mL SGN medium in test tubes to an initial OD600 of 0.1, and then cultivated at 30 °C in a shaker incubator (180 rpm; BR-43FL; Taitec).

Cells were collected using a 10-µL loop, which was passed through 7 cm of bacteria spread, were fully grown on the culture plates, and then inoculated into 2 mL of fermentation medium in test tubes (23 mm internal diameter × 200 mm length).

Strains were patched on solid TAP medium plus arginine and inoculated into 1.2 mL liquid M-N medium in test tubes.

Then, a few blue ones were picked up and inoculated in 6 ml SCVY liquid medium in test tubes and incubated for 3 5 days.

The pre-culture was inoculated in 5 mL of flesh LB medium in testing tube to OD600 = 0.1 and incubated with shaking at 120 rpm at 30 °C for 24 h.

In this study, behavioral test was conducted and agar gel pellet was used as the food medium in the test.

For the first round of screening, one loop of 3-day-old yeast culture was suspended in 3 mL of mixed-sugar medium in a test tube, and incubated for 3 days at 28 °C, with reciprocal shaking at 300 rpm.

Briefly, 200 μl of sterile deionized water was added to all outer-perimeter wells of sterile 96 well plates to minimize evaporation of the medium in the test wells during incubation.

Aliquots of culture were diluted with fresh medium in glass test tubes to obtain an OD600 = 0.19, and supplemented with 100, 20, 10, or 2 µg of ovorubin, respectively; sterile buffer was used as control.

Briefly, 200 µl of sterile deionized water was added to all outer-perimeter wells of a sterile 96-well plate (Corning Incorporated, Corning, NY, USA) to minimize evaporation of the medium in the test wells during incubation.