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Freeze-dried (0.4 mL) microorganism was aseptically transferred into the broth media in test tubes, cultured and propagated anaerobically in the broth media at 37 °C in an incubator, and then used in the syngas fermentation process.
For crude extract enzyme assays, 5-mL CGIII cultures of C. glutamicum harboring the relevant plasmids were grown overnight at 30°C in 5 mL CGIII media in test tubes.
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For testing the effects of FAS inhibitors on bacterial growth, stationary 16 h 37°C cultures of R. equi in BHI or of M. smegmatis in LB broth were used to inoculate 3 ml of the respective media in glass test tubes with 0.001 OD600 equivalents of bacteria.
High-efficiency particulate air filter application involves three steps: filter media testing, filter testing, and in-place filter testing.
For ecotoxicological assessment of ZnO and other nanomaterials, our approach has to be adapted despite media in ecotoxicological tests differ in their composition compared with cell culture media.
Almost all media in nanotoxicity tests contain both organic molecules and mineral ions, and their coadsorption on NP surface warrants discussion.
The majority of characterized creA mutations, including a precise deletion of the creA gene, abolish both acetate and glucose mediated repression when assayed on 1% glucose or 50 mM acetate media in standard tests for loss of carbon repression for genes subject to carbon repression.
To demonstrate that the neutralizing solution (Butterfield's Phosphate Buffer solution with surfactants, BBP++) and media used in testing and plating were capable of adequately neutralizing the antimicrobial properties of the antibacterial treated glove material, neutralization verification was performed using all four challenge bacterial species.
Effluents of the STPs were collected and used as test media in chronic toxicity tests with H. azteca.
This corresponds with the dose-dependent cytotoxicity of the conditioned media in the growth test (see Figure 4).
The prepared solutions were adjusted to pH 5.0 and used as media in the growth test of the fermenting yeast, S. cerevisiae CEN.PK 113-7D.
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