Finally, a MMN medium composition of pH 5.0 with 0.5% glucose, 1.0% peptone or tryptone and either (0.1%) sodium, potassium or manganese sulphate is adequate for the production of endoglucanase from ERM and DSE fungi.
This artificial neural network supported genetic algorithm predicted a maximum glucansucrase activity of 6.92 U/ml at medium composition of 0.54% (v/v) Tween 80, 5.98% (w/v) sucrose and 1.01% (w/v) K2HPO4.
Additional validation runs were adopted using the predicted optimal medium (medium composition of run no. 6) with 1 g L−1 as glucose was not a significant factor on the decolorization process and under the predicted optimal pH value (8) as pH significantly increased the decolorization process.
The medium composition of the two batches was: 80 g glucose, 7.5 g yeast extract, 30 ml corn syrup, 10 g KH2PO4, 0.5 g MgSO4, 0.01 g MnSO4, 5 g NH4Cl, 10 g Monosodium glutamate (MSG) per liter distilled H2O, pH 7.5.
In this study, the medium composition of ABM was optimized using response surface methodology for maximum mycelial biomass and extracellular polysaccharide (EPS) production.
C. Han investigated the medium composition of ABM that was optimized using response surface methodology for maximum mycelial biomass and extracellular polysaccharide (EPS) production.
