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Second, response surface methodology based on central composite design was applied to explore the optimal medium composition in a 7-l stirred fermentor.

The medium composition in submerged fermentation has a significant influence on the yield and quality of the Monascus yellow pigments (Chen and Johns 1994; Yongsmith et al. 1993).

As oxygen supply is a critical parameter controlling growth and product formation in aerobic bioprocesses and used as a parameter for bioprocess scale up, the effects of oxygen transfer, in terms of volumetric oxygen transfer coefficient (kLa), on production of sonorensin was investigated using optimized medium composition in a bioreactor.

The differentiation medium was also concentrated up to 4000 X, using a cut-off filter of 3 kDa and served as control medium to check medium composition in this study.

Notably, the importance of correctly describing the medium composition in growth phenotype predictions has been raised in previous studies [ 26].

Accordingly, the toxic effects of nanoparticles show variation depending upon the medium composition in which the nanoparticles are suspended; in another way round, the same nanoparticles exhibit different toxic manifestations when dissolved in different mediums [ 79, 80].

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For the growth analysis, our control ("normal") medium contained NO3 −, Ca2+ and K+ at 10, 5 and 5 mM, respectively (see medium compositions in Additional file 1: Table S2).

In this study, we focused on the medium composition originated in freshwater fishes and sea fishes.

The false predictions might be due to missing information in gene regulation, biomass compositions, dead-end reactions and medium composition, especially in the rich medium [ 18, 19] * = Aerobic growth, ** = Anaerobic growth TP = true positive, TN = true negative, FP = false positive, FN = false negative.

Detail of medium composition is in Additional file 3: Table S11.

In order to increase ATP pool size, the culture medium composition was optimized in this study because gene manipulation technologies are not preferable for industrial food production.

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