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The strategy is based on the change of fermentation media composition in terms of nutrients, by the development of an experimental design directed to aromatic amino acids pathway.
Verification experiment using the optimum media composition in a continuous stirred tank reactor indicated a highly reproducible result in which the Ps of 2608 mL H2/L was achieved at a hydraulic retention time of 32 h.
The strategies were driven by the development of a four single-factor experimental design and were based on the change of culture media composition in terms of carbon and nitrogen and the modification of the pH control by using NaOH or NH4OH.
Microbial lipase production often depends on one or more of the following parameters: growth media composition (in particular the enzyme inducing compound), substrate concentration, growth rate, fermentation process design (i.e. batch, fed-batch or continuous operation mode) and interfacial area between lipid and water phase.
To the best of our knowledge this is the first study to evaluate functionality of porous collagen matrix and collagen gels for in vitro culture of fibroblasts while investigating the role of culture media composition in modulating morphology, phenotype, extracellular matrix (ECM -related gECM -relatedon and protein synthesis by fibroblasts.
The constraining conditions of the simulation (listed as media composition in Additional file 6), were derived by literature mining to identify a media composition that could closely mimic nutrient availability for S. typhimurium in the host cell.
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The cultivation media composition ranges in three major groups: complex, semi complex, and defined.
X-ray fluorescence method was used for investigation of the liquid media composition changes in which the concrete samples were exposed.
This variability observed in BAT mitochondria may depend on the assay media composition or, perhaps, in the degree of uncoupling of the mitochondria used [ 25, 26].
After having optimized the incubation parameters (concentration, incubation time, and media composition) as described in Sections 3.1 and 3.2, one can proceed in analysis of the photoinduced cytotoxic effects of a new PS.
BG11 growth media composition as mentioned in Appendix A-8 was also added into the synthetic solution.
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