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Exact(6)
The nanoparticles were dispersed in the medium at concentration of 100, 250 and 500 (g/ml).
To visualize chromosomes, Hoechst33342 (Sigma) was added to the medium at concentration of 5 10 ng/ml.
Plant extracts were added to the top layer medium at concentration of 100 μg/mL.
5-Aza-dC (Sigma, St . Louis MO, USA) was added to the fresh medium at concentration of 1, 5, and 10 μM in duplicate and cells were harvested after 5 days.
For the assays, the extract was concentrated to dryness in a lyophilizer, the dried preparation was homogenized in culture medium at concentration of 10 mg/ml (stock solution), and filtered through a 0.45 μM Millipore.
To stain mitochondria for motility assay MitoTracker Red CMXRos or Deep Red FM (Invitrogen, USA) was added to the culture medium at concentration 15 ng/ml and the cells incubated for 30 min before this medium was removed and replaced with fresh medium.
Similar(54)
The sterile MNPs were dissolved in DMEM medium at concentrations of 20, 100, and 500 μg/mL.
The nanoparticles were well-dispersed in the culture medium at concentrations of 100, 250, and 500 μg/ml.
Reactivation yields were increased with respect to the control (fully aqueous medium) when cosolvents were added to the reactivation medium at concentrations below 50% (v/v).
Then, Lf-M-PAEEP-PLLA-NPs or M-PAEEP-PLLA-NPs was added in cell culture medium at concentrations of 5, 10, 15, and 20 μg/mL.
Similarly, MWCNT dispersed in a growth medium at concentrations of 10, 20 or 40 mg/L enhanced seed germination and biomass production [100].
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