Sentence examples for medium and selected from inspiring English sources

Transfected cells were cultured in their optimized medium and selected with puromycin (0.25 µg/ml) and hygromycin (100 µg/ml).

The J1 cells were selected in ES cell medium: 15% FCS in DMEM (Invitrogen, UK) supplemented with LIF (homemade) (ES cell medium) and selected wtih 110 µg/ml hygromycin (Invitrogen) 48 hours after electroporation.

HEK 293S cells were stably transfected with plasmids coding for LRP1b ectodomains II, III, and IV [3], grown in the same medium and selected with G418.

A low serum medium and selected mitogens were used for their known ability to encourage self-renewal by preventing in vitro commitment to differentiation, and to preserve the ability to form tumors in immunodeficient mice that recapitulate the heterogeneity normally seen on human cancers [ 16, 21].

We next compared the mRNA and protein profiles of cytokines in T cell medium, and selected those showing upregulation at mRNA level in activated T cells and detectable protein level in T cell medium, namely IL-1α, IL-2, IL-3, IL-10, IL-13, IFN-γ and TNF-α as the initial pool to start screening (Supplementary information, Figure S4B).

From the experimental data three selected pollutant parameters were tested to fit into different kinetic models for the adsorption rate, the removal mechanism and process and predict information about the interaction between these two naval adsorbents (filter medium) and those selected three pollutants (NO3 N, PO4 P and [Cr]T) in the tannery wastewater (Martins et al. 2013).

The parasite lines CS2 and CS2 transfected with a plasmid containing a green fluorescent protein (GFP) expression cassette (Wilson et al. in preparation) were maintained in RPMI-HEPES supplemented with 0.5% AlbumaxII (GIBCO) and 25mM of NaHCO3 (parasite medium) and gelatin selected (by flotation) every 2 weeks.

Forty-eight hours after transfection, the cells were washed with PBS and supplied with fresh medium and then selected with G418-rich medium (500 μg/mL).

Among these strains, two strains, 9-41A and M1-16 expressed the highest cholesterol-removing abilities in their growth medium and were selected for the present study.

The drug was administered to adult flies orally by supplementation in the food medium, and we selected the doses that produced small to essentially complete inhibition of egg production (1.56 μM to 156 μM) for lifespan assays.

The transformants were grown on double-dropout medium (DDO: SD/-Trp/-Leu) and selected on triple-dropout medium (TDO: SD/-Trp/-Leu/-His) containing 10 mM 3-amino-1, 2, 4-triazole (3-AT).