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Samples of growth medium and bacteria were taken over the 12 h time course.
Monolayers were washed with medium and bacteria were added to the cells at a multiplicity of infection (MOI) of 100, as for other end-point adhesion experiments.
Plates were incubated for 48 h as described above before the coverslips were removed, dip washed in (phosphate buffered saline PBS and added to new 24-well plates containing fresh medium and bacteria [30].
E. coli ATCC 25922 bacteria were grown to mid-logarithmic phase in TSB medium, and bacteria were washed and resuspended in 10 mM Tris, pH 7.4, 0.15 M NaCl, with 10 mM glucose, to yield a suspension of 1×107 cfu/ml.
The aim of this study is to determine whether the microbial metabolism of alkaliphilic bacteria can be harnessed for the reductive precipitation of Cr VI) in high-pH leachates of COPR, modified with haloalkaliphilic medium and bacteria.
From a culture grown overnight, a 4% (vol/vol) inoculum was transferred to fresh medium and bacteria were grown at 30°C until the optical density at 600 nm was 0.4.
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Paired log-rank test to evaluate the effect of growth medium and bacterium type on the lifespan of C. elegans.
Plates containing positive (bacteria with medium) and negative (bacteria with 10 mg/mL Streptomycin) controls for each bacterial species were also prepared.
A total of four subcultures from the colonies obtained from women were conducted on MRS Agar culture medium, and the bacteria that formed clear white colonies were selected.
In order to show the effect of the antibiotic on the bacteria inside the eggs, bacteria in streptomycin (0.5 mg/mL) treated eggs (5 eggs were grinded and diluted in 1 mL sterile water) were cultivated on the Luria Bertani culture medium, and the bacteria in normal eggs were cultivated as control.
For these experiments, H. influenzae was grown in μ-well chambers with sBHI medium and the bacteria were visualized with SYTO9 (see Materials and Methods).
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