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Exact(6)
The problem with the low-contrast region is that there are too little textures to apply the feature-based methods (such as median pixel value [22], gradient [23], and ZNCC).
For each fly we determined its median pixel value during the entire length of each 6-hour movie.
A median filter replaces the original pixel value by the median pixel value among the current pixel and its neighboring pixels.
While the median pixel value of the R545/R575 image ratio for normal/clinically healthy tissue was 0.87 (IQR = 0.82-0.94), they were 1.35 (IQR = 1.13-1.67) and 2.44 (IQR = 1.78-3.80) for pre-malignant and malignant lesions, respectively.
The fluorescence signal intensity was determined as the volume in a fixed-size circle, and background was estimated as the median pixel value in a diamond-shaped region between each adjacent four-spot region.
Spot intensity was computed as the integrated optical density or volume in a fixed-size circle; background intensity was computed as the median pixel value of four diamond-shaped regions at the corners of each spot.
Similar(54)
Common measures of expression include background subtracted mean or median pixel values measures for each arrayed gene.
The median pixel values of feature and background were used as the signal and background intensities, respectively.
Median pixel intensity values were used to represent protein levels instead of mean values as the latter are affected by both outliers and departure from normality.
Cy3 and Cy5 median pixel intensity values were background subtracted, normalized and subject to further analysis.
As with the AJ, a ratio of median pixel intensity values of expression domain over wild type cells was used to quantify changes in Armadillo and E-Cadherin levels.
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