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Additionally, the saccadic artifact at the right of Fig. 4(C) was removed by the median pixel selection.
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The idea behind choosing only the noise-free pixels is to avoid selection of a noisy pixel as the median pixel.
Each pixel contains a detector together with three transistors that are used for pixel reset, pixel selection and charge-to-voltage conversion.
The median pixel intensities were background subtracted.
The intensity of each spot was summarized as the median pixel intensity followed by log transformation.
Cy3 and Cy5 median pixel intensity values were background subtracted, normalized and subject to further analysis.
Following pixel selection, a fuzziness setting of 14 was used, and the chosen pixel number was calculated using the image histogram calculation.
For each fly we determined its median pixel value during the entire length of each 6-hour movie.
A median filter replaces the original pixel value by the median pixel value among the current pixel and its neighboring pixels.
Median pixel intensity of each spot was used for analysis.
For all analyses, the median pixel intensities for each spot were used.
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