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Media samples collected at day 7 were assayed for GAG release while collagen degradation and release was measured in media collected at days 7, 14, 21 and 28, all were stored at -20°C prior to assay.
As seen in the PCA score plot (Figure 3A), distinct differences in the metabolic profiles of media samples collected over time were observable.
Reference to the corresponding loadings plots showed that media samples collected at 24 h contain comparatively higher concentrations of glycolysis endpoints such as lactate, alanine and pyruvate than 48 h and 72 h time points.
A rapid analysis of metabolic changes of TeSR-1 media samples collected every 24 h for 3 days was achieved through the application of PCA to the NMR-acquired data.
Briefly, the media samples collected were filtered through 0.2 μm syringe filters.
Viral replication was assayed by real-time reverse transcription polymerase chain reaction using cell media samples collected on days 0, 4, and 7 postchallenge.
Similar(53)
The IAAF said Radcliffe was the victim of untrue allegations and was "hounded remorselessly in the media" after blood samples collected in Portugal in 2003, Finland in 2005 and Monte Carlo in 2012 were described as suspicious.
Media samples were collected from the cell culture dish, and 600 μl of the media sample was used for NMR analysis.
Media samples were collected at 1, 2, 4, 8, 24, and 48 hours.
To determine the effect of mutant REP-1 on secretion of cytokines and growth factors by the peripheral cells, conditioned media samples were collected from the primary fibroblast cultures of 8 CHM patients and 9 controls.
After 48 hrs of co-culture and tube formation, media samples were collected and tested.
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