Media samples were collected from the cell culture dish, and 600 μl of the media sample was used for NMR analysis.
But he added that other tests of media samples were also being conducted at the same time.
Media samples were collected at 1, 2, 4, 8, 24, and 48 hours.
These results suggest that voids in post-treatment media samples were filled or occupied with nutrients and particulates adsorbed onto the media surface or biofilms, thus leading to the reduction of BET specific surface area and pore volume.
The test media samples were diluted with aqua regia at a ratio of 1 10 (1 mL sample diluted with 9 mL aqua regia) and digested using a microwave (Discover SP-D, CEM, Matthews, NC, USA; max temperature 175 °C, max pressure 25 bar).
If necessary, media samples were diluted using DMEM/F12+.
For hProCpepMyc constructs, transfected cell lysates and media samples were analyzed by Western blotting with anti-myc antibodies.
In parallel, media samples were taken to track expression of construct 4 by PAGE and western blot (Fig. 5A, B).
Media samples were injected directly onto a PrincetonSPHER60 SAX 5 µm (250×4.6 mm) column and separated isocratically with 20 mM KH2PO4 as the mobile phase.
To determine the effect of mutant REP-1 on secretion of cytokines and growth factors by the peripheral cells, conditioned media samples were collected from the primary fibroblast cultures of 8 CHM patients and 9 controls.
Conditioned Media samples were digested with 0.5 1 units per ml of plasmin in 40 mM Tris-HCl, 2 mM CaCl2, 2 mM MgCl2, and 0.02% Tween-20 for 30 minutes at 37°C.
