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Exact(5)
Media samples were collected from the cell culture dish, and 600 μl of the media sample was used for NMR analysis.
A small amount of media sample was placed on the ZnSe/diamond crystal.
The total nitrogen in the media sample was determined by distillation in Kjeldahl's apparatus and titration was carried out with standard H2SO4.
In blank, the media sample was replaced with de-ionized (DI) water.
The media sample was further examined to ensure that each article either made direct reference to the DFSS or made indirect reference to the study by presenting at least four study characteristics (e.g., year, journal, author, author affiliation, subjects, methods, results, limitations, ethical issues raised) that clearly established its identity.
Similar(55)
In triplicate, 40 µl of each standard and 40 µl from each media sample were added to a 96-well plate.
Immunoprecipitation of media samples was done using Protein-G agarose (Invitrogen).
The amount of glucose present in the media samples was determined using the Glucose Assay Kit (GAGO-20) from Sigma.
The mAb concentration in the different media samples was quantified by ELISA (Montgomery, TX, US) and UV vis spectroscopy using a Nanodrop Lite system (Thermo, Wilmington, DE, USA).
The amount of lactate present in the media samples was determined by generally following the Sigma Diagnostics Procedure No. 826-UV.
Then 50μl cell lysate samples or 10 μl of cell media samples was added to the 96-well plate along with a either a media or RIPA buffer blank.
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