Media samples were collected from the cell culture dish, and 600 μl of the media sample was used for NMR analysis.
A small amount of media sample was placed on the ZnSe/diamond crystal.
The total nitrogen in the media sample was determined by distillation in Kjeldahl's apparatus and titration was carried out with standard H2SO4.
In blank, the media sample was replaced with de-ionized (DI) water.
The media sample was further examined to ensure that each article either made direct reference to the DFSS or made indirect reference to the study by presenting at least four study characteristics (e.g., year, journal, author, author affiliation, subjects, methods, results, limitations, ethical issues raised) that clearly established its identity.
But he added that other tests of media samples were also being conducted at the same time.
Human TNF alpha Uncoated ELISA (Invitrogen-88-7346-22) waccordingmed according to manufacturer's instructions, media samples were diluted 1 50 and 100 µL was used.
These results suggest that voids in post-treatment media samples were filled or occupied with nutrients and particulates adsorbed onto the media surface or biofilms, thus leading to the reduction of BET specific surface area and pore volume.
Media samples were collected at 1, 2, 4, 8, 24, and 48 hours.
The test media samples were diluted with aqua regia at a ratio of 1 10 (1 mL sample diluted with 9 mL aqua regia) and digested using a microwave (Discover SP-D, CEM, Matthews, NC, USA; max temperature 175 °C, max pressure 25 bar).
