Sentence examples for media lacking either from inspiring English sources

Transformants were selected on synthetic complete (SC) media lacking either leucine (bait vector) or tryptophan (prey vector).

We therefore grew each of the cell lines to approximately 80% confluency under non-limiting conditions, then changed to media lacking either serum (Figure 3a) or glutamine (Figure 3b), and subsequently assessed viability.

The activation domain strain contains three different Gal4p-responsive reporter genes: GAL2-ADE2 and GAL1-HIS3, which are assayed by selection on yeast synthetic media lacking either adenine or histidine, respectively, and GAL7-lacZ, which can be monitored using colorimetric or luminescent assays for beta-galactosidase activity.

Two-hybrid protein interactions were evaluated by growing the yeast colonies at 28°C on media lacking either histidine or adenine and supplemented with different amounts of 3-amino-1,2,4-triazole (3-AT).

Transformations were plated on selective dropout media lacking either leucine, tryptophan and histidine supplemented with 0.5 m m 3-AT (for suppression of 'leaky' histidine expression) for nutritional selection, or leucine and tryptophan for transformation controls.

Co-transformed yeast was plated onto minimal media plates lacking either leucine and tryptophan (LT-, as a control for transformation efficiency) or adenine, histidine, leucine and tryptophan (AHLT-, as a test for interaction).

Log phase cells were normalized and plated on media lacking uracil and containing either glucose or galactose as the carbon source.

This strain was sporulated and the resulting spores were released from the ascus as described above and plated on either media lacking uracil or leucine.

Cell treatments were completed using media lacking serum and containing either 1 μg/mL hydrocortisone (HC) (Sigma), 10 μM RU-486 (Sigma), or ethanol vehicle for 48 hours.

Growth was conducted in liquid rich media (1% yeast extract, 2% peptone, 2% dextrose, YPD) for deletion strain growth curve assays, and liquid synthetic complete media lacking uracil (SC-ura) using either 2% dextrose or 2% raffinose as a carbon source for pre-growths of the overexpression strain for growth curve assays (detailed below).

After harvesting the cells by centrifugation, cells were washed twice with media lacking arabinose and used to inoculate cultures containing either 0.2% arabinose to induce FtsY expression or 0.2% fructose for FtsY depletion.