Media was aspirated, and the cells were incubated with fresh media containing either CAHA-sSWCNTs or sSWCNTs or CAHA at various concentrations along with control cells for 48 h.
Figure 2 shows the ratios of each metabolite in media containing either NaHCO3 or NaH13CO3.
Media was removed and replaced with media containing either test compounds (100 μM) or DMSO (0.1 %) for 24 h.
The growth rates of DST160 in media containing either 0.61 M NaCl, 0.61 M KCl, or at pH 4.5 were 25% higher, 18% lower, and 57% higher than those of wild-type, respectively, implying DST160 acquired salt tolerance and pH shock tolerance as well as succinate tolerance.
ES media containing either dorsomorphin or DMSO was changed daily for three days.
Following removal of the transfection mix, media containing either 150 nM of taxol (Paclitaxel) or 300 nM of nocadazole was added to the transfected cells.
Therefore the medium that contained SmAQP-suppressed worms simply looked redder than media containing either of the control groups of worms.
Cells were split the following day onto 10 cm plates with charcoal-absorbed sera media containing either 1 µM RA or DMSO.
Media was replaced at 36 h post-transfection with media containing either 75 µM chloroquine, 100 µM N-acetyl-leucinyl-leucinyl-norleucinal (ALLN, Sigma), or PBS (controls).
ULTI cells were seeded into 60 mm plastic dishes at a density of 1×105 cells/dish in complete media containing either 10% or 0.5% FBS, both with and without the addition of 10 U/ml IFN-γ.
No advantage for the E4 isolate compared to the knockout strain was observed in media containing either hemin or holo-transferrin as the main iron source (Figure 2C D).
