Sentence examples for media in triplicate from inspiring English sources

In brief, E520 neurospheres were dissociated and plated in quadruplicate in a 96-well plate (BD Biosciences) in 100 μL of stem cell media in triplicate.

After 24 hr growth, each plate was again pinned onto chemical-containing media in triplicate leading to nine total replicates passaged on YPD + chemical.

Cells were plated under standard serum conditions (10% FBS) in their respective media in triplicate wells/condition in 96-well flat bottom plates (Optilux, Falcon, Becton-Dickinson).

For assay of colony formation in soft agarose, cells were embedded in 0.33% agarose, which was sandwiched between a 0.6% agarose base and a 0.33% top layer with media, in triplicate in six-well plates.

NP cells were treated with IL-6 and TNFα at 0, 10 10 and 100 ng/ml in serum-free media in triplicate for three independent patients (Table  1).

Seedlings (inoculation experiments) or 0.7 cm3 excised leaf discs (infiltration experiments) were homogenized in 20% glycerol in 10 mM MgCl2, and appropriate dilutions of homogenates were plated on selective LB agar media in triplicates.

In brief, cells were treated with various concentrations of GSE in fresh media in triplicates.

5 μl of overnight TSB cultures of both microorganisms incubated at 37°C were used to inoculate each of the media plates in triplicate.

Protein concentration (μg/ μl) assessed in 5  μl conditioned media (performed in triplicate) using the Lowry protein assay kit (Bio-Rad).

The samples in each culture media were performed in triplicate.

HUVEC grown in complete media were seeded in triplicate in 8-well chamber slides coated with complete Matrigel (4×104 cells/well).